Skip to main content
. Author manuscript; available in PMC: 2018 Jul 31.
Published in final edited form as: RNA Biol. 2009 Jan 5;6(1):84–89. doi: 10.4161/rna.6.1.7572

Figure 3.

Figure 3

Cleavage/reverse transcription products determine areas of single- and double-strandedness. Sequencing and cleavage/RT reactions were carried out with a primer complementary to the 3′ end of the p27Kip1 5′-UTR and run on an 8% urea-containing polyacrylamide gel. Sample area demonstrates the results seen in the area of the human p27 Kip1 5′-UTR polypyrimidine tract. Arrows on the left point out G’s in the sequence that are cleaved by RNase T and are therefore single-stranded. Arrows on the right point out C’s and T’s in the sequence that are cleaved by RNase A and are also therefore single-stranded.