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. Author manuscript; available in PMC: 2018 Jul 31.
Published in final edited form as: RNA Biol. 2009 Jan 5;6(1):84–89. doi: 10.4161/rna.6.1.7572

Figure 5.

Figure 5

Ribosome entry window for IRES-initiated translation is between 36 and 50 nucleotides upstream of the p27Kip1 start codon. DNA transfections of MCF7 cells with the dual luciferase constructs containing the 472 base 5′-UTR sequence with the mutated upstream AUGs were done with GenePorter transfection reagent. Cells were harvested one day after transfection followed by assays for Renilla and firefly luciferase activity levels (in relative light units) and calculation of the firefly/Renilla ratio. Asterisks indicate statistically significant reduction in firefly/Renilla ratio relative to the wild-type control (p < 0.001).