Figure 7. n-cadherin (cdh2^vu125) mutants exhibit both oval and divided pineal phenotypes.

(A – C) Confocal images of the immunofluorescence labeling of foxd3:GFP positive embryos at 2 dpf. foxd3:GFP is expressed in the pineal and parapineal organs (white arrowheads). Axonal projections from parapineal neurons are denoted with open arrowheads. (D – F) Two color in situ hybridization showing parapineal cells expressing gfi1.2 (blue) and pineal cells expressing flh (red) in 2 dpf embryos. The dashed lines represent the embryonic midline. (A, D) WT embryos have a fused, oval pineal organ and a single left sided parapineal organ. (B, E) Approximately half of the cdh2 mutants with a divided pineal organ have only a single parapineal organ. (C, F) Approximately half of the cdh2 mutants with a divided pineal organ have two bilaterally located parapineal organs. All images are dorsal views with representative images shown. All scale bars = 20 μm. (G) Graph of parapineal placement in WT siblings (n = 261), cdh2 mutants with oval shaped pineal (n = 46) and cdh2 mutants with divided pineal (n = 114).