Figure 3.

USP8 promotes proliferation, migration, and invasion of CSCC cells. The transfection efficiencies of USP8-overexpression and USP8-siRNA were tested in SiHa (A) and SW756 (B) cell lines by Western blot analysis. After verifying the transfection efficiency, transfected cells were enrolled into proliferation assay by CCK-8 method (C, D), migration assay by Transwell (E, F), and invasion assay by Matrigel-Transwell (G, H), respectively. The detailed assay procedures are described in the Methods section. Tumor biological data showed that USP8 overexpression has little effect on cell proliferation, but significantly promotes the migration and invasion of both SiHa and SW756 cells, whereas USP8-siRNA showed the opposite effects.