Figure 3.

Phenotypes of Grafted hPESC-Derived DA Neurons 9 Months Post Grafting in MPTP-Induced PD Monkey Hosts

(A) Grafted neurons were labeled by Perl's Prussian blue staining. Robust survival and migration of dopamine neurons in the host brains were observed. The boxed area in each image is shown at a higher magnification on the right. Arrowheads indicate grafted neurons. Scale bar, 20 μm.

(B) Histological analysis using DAB-developed immunohistochemistry for tyrosine hydroxylase (TH) revealed numbers of TH⁺ neurons dispersed throughout the graft, which is indistinguishable between the EB-DA and FP-DA groups. Scale bar, 200 μm.

(C and D) Expression of TH (C, green; scale bar, 20 μm) and human-specific neural marker NCAM (D, green; scale bar, 50 μm) co-localized with rhodamine (Rho, red).

(E) The number of hNCAM⁺ cells and TH⁺ cells surviving in each monkey. Data are presented as mean ± SEM, n = 3.

(F) Immunostaining for TH (red) and co-expression (green) with GIRK2. Scale bar, 20 μm.

(G) The percentage of GIRK2⁺ cells per TH⁺ DA neuron. Data are presented as mean ± SEM, n = 3.

See also Figure S3.