Figure 4.

IFNγ and Canonical Wnt Signaling Pathways Stimulate Osteogenic and Inhibit Adipogenic Differentiation of Skeletal Progenitors

(A) Enrichment of osteo- and adipogenic transcriptional signatures for members of signaling pathways according to the RNA profiling.

(B) A set of signaling molecules was tested for the effect on the osteogenic and adipogenic induction of the “O” (top) and “A” (bottom) mouse BMSC clones.

(C) Dose dependence of the effect of signaling molecules on the differentiation properties of mouse clonal skeletal progenitors. Osteogenesis was quantified by elution of Alizarin Red dye and measurement of OD₄₉₀, and adipogenesis was assessed by counting the proportion of adipocytes.

See also Figure S3.