Figure 2.

Elevated Nox4 mRNA in the PCa‐associated stroma is associated with epithelial TGFβ expression. Dual Nox4 in situ hybridization and TGFβ immunohistochemistry (pan TGFβ antibody) on radical prostatectomy prostate tissue from PCa patients. Two cancer (CA) and one benign (BE) adjacent areas are shown. Single and multiple Nox4 mRNA transcripts appear as single red dots or clusters, respectively. Yellow/brown chromogen staining for TGFβ. Original magnification: top panel 20×, middle and bottom panels 40×. Images are representative of 3 independent experiments using tissue isolated from different patients. Middle right panel: *, benign gland expressing very low levels of TGFβ and little stromal Nox4 with adjacent TGFβ‐positive tumor cells (#) surrounded by abundant Nox4 expression in the tumor‐associated stroma. Black and red boxed regions are enlarged in the middle and lower panels, respectively. Images of parallel‐stained negative controls are depicted in Supporting Information, Figure S6.