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. 2018 Mar 1;143(2):383–395. doi: 10.1002/ijc.31316

Figure 5.

Figure 5

GKT137831 attenuates paracrine‐mediated induction of PCa cell proliferation and migration. Primary human prostate fibroblasts were treated with bFGF or TGFβ1 in the presence of GKT137831 (GKT) or DMSO equivalent (control, ctrl) for 48 hr. Cells were washed with serum‐free media and further incubated in serum‐free media in the presence of GKT137831 or DMSO equivalent for 48 hr before collection of conditioned media (CM). (a) Fibroblast CM or non‐CM (serum‐free DMEM containing 30 µM GKT137831 or DMSO equivalent incubated without fibroblasts) was applied to the indicated PCa cell line for 96 hr before analysis of proliferation via SybrGreen assay. (b,c) CM or non‐CM (serum‐free DMEM containing 30 µM GKT137831 or DMSO equivalent incubated without cells) was used as chemoattractant in the lower chamber of transwell migration assays for (b) CWR22Rv1 or (c) DU145 cells seeded in serum‐free media in transwell inserts and migrating cells analyzed (b) 24 hr or (c) 48 hr later. (a–c) Data represent mean + SEM of three independent experiments using CM harvested from primary fibroblasts isolated from different donors. Statistical significance is depicted (**p < 0.01; ***p < 0.001). Representative images are shown.