Time course of wnt3a, Frz1, PIWI1a, pGsk-3β, FOXM1, and CC-3 in the right hemisphere of the rat brain after MCAO. A, Representative Western blots. Quantitative analysis with Western blot showed that the expression of (B) wnt3a and (C) Frz1 significantly decreased by 6 h. D, PIWI1a was decreased by 12 h and returned to sham levels by 72 h. E, However, pGsk-3β was increased after MCAO. F, FOXM1 was decreased after MCAO, but (G) CC-3 was increased. Each column represents the mean ± SD (n = 4/group). *p < 0.05 versus Sham. #p < 0.05 versus 6 h. Figure 2-1 reports the specific statistics for each group comparison.