Figure 5.

Expression of Frz1 (red) in neurons (NeuN, green), astrocytes (GFAP, green), and microglia (Iba1, green) in sham,vehicle, and treatment. A, Representative triphenyl tetrazolium chloride stained coronal section showing the location of all immunohistochemistry images indicated by the boxed region. B, Expression of Frz1 decreased in vehicle at 24 HR after MCAO in neurons but was increased with treatment. Sham showed minimal expression of Frz1 in astrocytes and no expression in microglia, whereas vehicle and HD treatment (1.2 ug/kg) had no Frz1 expression in astrocytes and microglia (n = 1 per each group). Blue represents DAPI. Scale bar, 50 μm.