Figure 4.

Effect of bilateral microdialysis administration of ZD7288 in the VB on GHB-elicited ASs in freely moving Wistar rats. A, Position of the bilateral microdialysis probes (black thick lines) and diffusion areas of ZD7288 (red circled, striped area) are depicted on a rat brain schematic drawing at the level of the VB [modified from Paxinos and Watson (2007)]. B, Representative EEG traces showing GHB-elicited SWDs during ACSF and ZD7288 (500 μm in the inlet tube) administration. C, Left, Effect (mean ± SEM) of ZD7288 (500 μm, filled bars, n = 11 rats) versus ACSF (open bars, n = 7) on total time spent in seizures illustrated for 10 min bins. ZD7288 dialysis started 40 min before GHB injection (see Materials and Methods for further details). Right, Individual data points (open, ACSF; filled, ZD7288) for the 0–10 and 10–20 min time bins after GHB injection are normalized to data recorded during ACSF injection (**p = 9.4 10⁴, *p = 0.013, Wilcoxon rank-sum test, n = 7 and 11 animals in each group). D, E, Similar bar graphs (left) and scatter plots (right) as in C for average length of individual seizures (D; *p = 0.016, **p = 0.0082, Wilcoxon rank-sum test, n = 7 and 11 animals in each group) and number of seizures (E; p = 0.1, p = 0.34, Wilcoxon rank-sum test, n = 7 and 11 animals in each group).