Figure 2.

Infiltrating M2 macrophages in the parasite-infected brains express galectin-3 in the cytosol, but not on cell surface. Mice were injected intracranially with HBSS (mock) or 60 helminth parasite (infected), and brain mononuclear cells were isolated at 3 weeks after inoculation. A, Schematic for gating CD45⁺F4/80⁺ (macrophages/microglia) cells that express galectin-3. B, Representative scatter plots from 3 independent experiments showing percentage of CD45^hiF4/80⁺ (infiltrating macrophages) or CD45^intF4/80⁺ (microglia) cells from the brains of mock-infected and parasite-infected mice exhibiting intracellular or cell surface expression of galectin-3. C, Galectin-3 expression was quantified in CD45^hiF4/80⁺ macrophages or CD45^intF4/80⁺ microglia from 3 independent experiments. CD45hiF4/80⁺ GAL 3⁺ cells intracellular (n = 6); CD45^hiF4/80⁺ GAL 3⁺ cell surface (n = 6); CD45i^ntF4/80⁺ microglia intracellular (n = 6); CD45i^ntF4/80⁺ microglia surface (n = 6). p values (t test).