Figure 3.

PGE₂ potentiates primary afferent-evoked EPSCs in mature lamina I projection neurons via presynaptic mechanisms. A, Representative plot of current versus time illustrating a slight increase in EPSC amplitude in response to the bath application of PGE₂ (10 μm). B, PGE₂ significantly increased monosynaptic EPSC amplitude in adult projection neurons (n = 7; *p = 0.0156; Wilcoxon signed-rank test). C, Examples of EPSCs evoked by paired stimulation in the absence (black) and presence (gray) of PGE₂. D, The PPR was significantly decreased by PGE₂ (n = 7; p = 0.006; repeated-measures 2-way ANOVA; **p < 0.01; Bonferroni post-test), suggesting an elevation in the probability of glutamate release from the terminals of sensory afferents.