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Validation of heavy isotope labeled Ac3GalNAc-Bn O-glycan precursor. MALDI-TOF-MS analysis of unlabeled Ac3GalNAc-BnH7 (A) and heavy labeled Ac3GalNAc-BnD7 (B) O-glycan precursors. (C) Representative spectra or (D) summary data (n = 4) of heavy and light labeled precursors mixed 1:1. Two-tailed t-test performed for D; A,B repeated two-independent times.
