Fig. 5.

ICORA detects changes in the relative abundance of O-glycans. Heavy and light 50 μM Ac₃GalNAc-Bn O-glycan precursors were separately incubated with HEK-293 cells. Media from the cells were mixed in 1:9, 1:3, 1:1, 3:1 and 9:1 ratios. L:H ratios were normalized to L:H of 1:1 mixing and plotted as summary data (n = 3) (A) or shown as representative individual spectra for disialyl core 1 (B).