Figure 2.

Characterization of EpiSC^S/F

(A) Expression of SOX2 and OCT4 (upper panels), NANOG, FGF5, and T (middle panels), and OTX2, SIX3, and E-CADHERIN (lower panels) in EpiSCs^S/F and EpiSCs. Immunofluorescence and DAPI counterstaining. ^∗p < 0.05, ^∗∗∗p < 0.001, significant difference in the fraction of cells displaying positive immunofluorescence (% positive/DAPI) by Student's t test, n = 5 samples for each type of EpiSC. n.s., not significant. Scale bars, 50 μm.

(B) qPCR analysis of the expression of Oct4 and Otx2 in EpiSCs and EpiSCs^S/F (at passages 23 and 30). Thirty cells per cell type were analyzed by qPCR. Statistical analysis was performed using Student's t test (^∗∗∗p < 0.001; n.s., not significant).

Data are means ± SD.