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. 2018 Jun 28;11(1):43–57. doi: 10.1016/j.stemcr.2018.05.019

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Developmental Correlates of EpiSCs^S/F

(A) Gene ontology (GO) analysis of differentially expressed genes (DEG) in EpiSC and EpiSC^S/F.

(B) Epiblast/ectoderm samples for RNA-seq from E5.5 (cavity stage, Cav), E6.0 (prestreak stage, PS), E6.5 (early-streak stage, ES), E7.0 (late-middle streak stage, LMS), and E7.5 (early-bud stage, EB) mouse embryos. A, anterior; P, posterior; Epi, epiblast; AP, anterior proximal; AD, anterior distal.

(C) Hierarchical clustering by the transcriptome of EpiSCs^S/F (as technical replicates, red), EpiSCs, ESD-EpiSCs, and epiblast/ectoderm samples.

(D) Pearson correlation analysis of EpiSCs^S/F and EpiSCs with embryonic tissue samples for matching the EpiSC and EpiSC^S/F to the epiblast/ectoderm of E5.5 to E7.5 embryos.