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. 2015 Sep;43(9):1372–1380. doi: 10.1124/dmd.115.065193

Fig. 3.

Fig. 3.

N-oxidation of TMP to primary metabolites. (A) 1- and 3-NO-TMP formation by human cDNA-expressed P450 enzymes. TMP (5 µM) was incubated with cDNA-expressed P450 enzymes as described under Materials and Methods. Rates shown are corrected for rates from microsomes containing vectors alone. Each bar represents the mean of the duplicate incubations. (B) Sample-to-sample variation in the rates of TMP 1- and 3-N-oxidation by HLMs at a substrate concentration of 5 µM. Each bar represents the mean of the triplicate experiments ± S.D. (C) Effects of various P450 isoform–selective inhibitors on the formation of 1- and 3-NO-TMP by pooled HLMs at a substrate concentration of 50 µM, as described under Materials and Methods. Each bar represents the mean of the triplicate incubations. Uninhibited mean rates of 1- and 3-NO-TMP formation were 8.2 and 1.6 pmol/mg protein/min, respectively.