TABLE 1.
Primers/Probes | Sequence |
---|---|
For total CYP3A5 | |
CYP3A5 Ex 1 forward | 5′-GACCTCATCCCAAATTTGGCGG |
CYP3A5 Ex 4 reverse | 5′-CAGGGAGTTGACCTTCATACGTT |
For normally spliced CYP3A5 | |
CYP3A5 Ex 2/3 forward | 5′-CCTATCGTCAGGGTCTCTGGAA |
CYP3A5 Ex 4 reverse | 5′-TGATGGCCAGCACAGGGA |
CYP3A5 Ex 3/4 probe | 5′-[6FAM]ATGTGGGGAACGTATGAA[BHQ1] |
For CYP3A5 SV1 | |
CYP3A5 Ex 3B forward | 5′-GTGAGACTCTTGCTGTGTGTCACA |
CYP3A5 Ex 3B/4 reverse | 5′-GGAGTTGACCTTCATACGTTCCC |
CYP3A5 Ex 3B probe | 5′-[6FAM]CTGTGTGTCGTACAACT[BHQ1] |