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. 2015 Aug;43(8):1286–1293. doi: 10.1124/dmd.115.064998

TABLE 1.

Primers and probes for quantitation of CYP3A5 transcripts

Primers/Probes Sequence
For total CYP3A5
 CYP3A5 Ex 1 forward 5′-GACCTCATCCCAAATTTGGCGG
 CYP3A5 Ex 4 reverse 5′-CAGGGAGTTGACCTTCATACGTT
For normally spliced CYP3A5
 CYP3A5 Ex 2/3 forward 5′-CCTATCGTCAGGGTCTCTGGAA
 CYP3A5 Ex 4 reverse 5′-TGATGGCCAGCACAGGGA
 CYP3A5 Ex 3/4 probe 5′-[6FAM]ATGTGGGGAACGTATGAA[BHQ1]
For CYP3A5 SV1
 CYP3A5 Ex 3B forward 5′-GTGAGACTCTTGCTGTGTGTCACA
 CYP3A5 Ex 3B/4 reverse 5′-GGAGTTGACCTTCATACGTTCCC
 CYP3A5 Ex 3B probe 5′-[6FAM]CTGTGTGTCGTACAACT[BHQ1]