TABLE 3.

In vitro activity of recombinant CYP3A enzymes toward midazolam

rCYP3A5	rCYP3A7	rCYP3A4	Midazolam Metabolite Formation		1ʹ-OH MDZ/4-OH MDZ Ratio
			1ʹ-OH MDZ	4-OH MDZ
pmol			pmol/min/pmol P450
—	—	2.5	4.20	1.04	4.05
2.5	—	—	10.16	1.15	8.85
—	2.5	—	0.72	0.70	1.03
2.25	0.25	—	6.86	0.91	7.52
1.875	0.625	—	6.62	1.00	6.65
1.25	1.25	—	4.20	0.85	4.96
0.625	1.875	—	2.38	0.74	3.22
0.25	2.25	—	1.20	0.66	1.82