TABLE 1.
Summary of subcellular kinetics: Vmax and Km for each metabolite in liver microsome, S9, and cytosolic fractions
| Liver Microsome |
Liver S9 |
Liver Cytosolic |
||||
|---|---|---|---|---|---|---|
| Vmax | Km | Vmax | Km | Vmax | Km | |
| pmol/min per milligram | μM | pmol/min per milligram | μM | pmol/min per milligram | μM | |
| HBUP | 131.2 ± 5.8 | 87.9 ± 20.2 | 51.4 ± 1.9 | 99.5 ± 18.9 | 1.5 ± 0.5 | 71.3 ± 127 |
| TBUP | 98.4 ± 6.6 | 186.3 ± 53.5 | 99 ± 7.5 | 265.7 + 77.7 | 14.5 + 0.7 | 89.8 ± 22 |
| EBUP | 2.6 ± 0.3 | 41.4 ± 26.6 | 4.2 ± 0.28 | 107 ± 32.1 | 3.65 ± 1.2 | 274 ± 254 |
HBUP, hydroxybupropion; TBUP, threohydrobupropion; EBUP, erythrohydrobupropion.