TABLE 3.
Estimated intrinsic clearance
Using eq. (2) the intrinsic clearance for both liver and intestinal S9 fractions was calculated on the basis of the Michaelis Menten equation, assuming the linear portion of the curve. The relative contribution for each metabolite in S9 liver or intestines is indicated in the table.
| Subcellular Fraction | Metabolite | Clint |
|---|---|---|
| Liver S9 fraction | Hydroxybupropion | 519 μl/min per milligram |
| Threohydrobupropion | 372 μl/min per milligram | |
| Erythrohydrobupropion | 39 μl/min per milligram | |
| Total Clint from liver | 931.8 μl/min per milligram | |
| Intestinal S9 fraction | Hydroxybupropion | N/A |
| Threohydrobupropion | 45 μl/min per milligram | |
| Erythrohydrobupropion | N/A | |
| Total Clint from intestines | 45 μl/min per milligram | |
| Total contribution from liver and intestinal S9 fractions | 976 μl/min per milligram |