Figure 2.

The Two Types of Cells Are Both Pluripotent

(A) The images indicated that both MCoG and DCoG cells formed colonies and expressed AP.

(B) NANOG and OCT4 were expressed both in MCoG and DCoG cells after 26 passages.

(C) RT-PCR analysis of expression of pluripotency genes (OCT4 and NANOG) and differentiation genes (PAX6, ectoderm; BRACHYURY, mesoderm; and AFP, endoderm) in MCoG and DCoG cells.

(D) Flow cytometric analysis of pluripotency markers in MCoG and DCoG cells. The 50,000 cells analyzed express high levels of hPSC-specific cell surface markers (TRA-1-60, SSEA-4) and low levels of differentiation-specific cell surface markers (SSEA-1).

(E) Embryoid bodies formed by MCoG and DCoG cells differentiated into the three germ layers: ectoderm (β-tubulin), mesoderm (α-SMA), and endoderm (SOX17 and AFP).

(F) Teratomas formed by MCoG and DCoG cells in severe combined immunodeficiency mice, containing tissues that are representative of all three embryonic germ layers.

(G) Normal karyotype exhibited in both MCoG and DCoG cells.

See also Figure S3.