Fig. 6.

Unidirectional fluxes [aqueous humor-to-blood (AH-to-blood); blood-to-aqueous humor (blood-to-AH)] and net active flux of PAH across bovine ciliary body in Ussing chambers (A and B). (A) Representative time-course experiment (n = 1). (B) Fluxes at steady state (t = 2 hours) from four separate experiments (mean ± S.E. of the mean). The unlabeled concentration of PAH used was 1 mM. Net flux is the difference between the unidirectional fluxes and is in the AH-to-blood direction. *P < 0.05, significantly different from the AH-to-blood flux, two-tailed unpaired Student’s t test. (C) mRNA expression analysis of Oat1, Oat3, Nadc3, and Mrp4 in the bovine ciliary body (CB) by RT-PCR. Kidney was used as a positive control (kid). Polymerase chain reaction products were separated on 1% agarose gels and visualized with ethidium bromide.