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. Author manuscript; available in PMC: 2018 Jul 31.
Published in final edited form as: Bio Protoc. 2018 Jul 20;8(14):e2940. doi: 10.21769/BioProtoc.2940

Figure 7. HSF-1::GFP nuclear foci formation upon heat shock.

Figure 7

A. Transgenic EQ87 iqIs28 [hsf-1::gfp] L4 C. elegans without heat shock, imaged with the single band filter set (left panel) and the triple band filter set (right panel). Anterior to the top, ventral side left. 1/30 sec exposure time). B. Head region of a transgenic animal without heat shock, imaged at a higher magnification with the single band filter set (left panel) and the triple band filter set (right panel; 1/55 sec exposure time). C. With a 20 min heat shock at 35 °C, imaged with the single band filter set (top panel) and the triple band filter set (bottom panel; 1/30 sec exposure time). D. Head region of a transgenic animal with a 20 min heat shock at 35 °C, imaged at a higher magnification with the single band filter set (left panel) and the triple band filter set (right panel; 1/55 sec exposure time). For (A) and (C), images were stitched together using ImageJ (https://imagej.net/Image_Stitching) (Preibisch et al., 2009).