FIG. 4.

Specificity of EcDsbA inhibitors for two diverse DsbA homologues in UPEC. UPEC strain CFT073 encodes DsbA and DsbL, both of which can complement the motility defect of the 2KO mutant when provided in trans (2KO + pUC19 empty vector vs. 2KO + pDsbAB/pDsbLI), fully or partially, respectively. Restoration of CFT073 2KO motility by either DsbA or DsbL was inhibited by all four inhibitors at a similar level. Dot plots represent mean motility zone diameter (mm) ± SEM of four independent replicates for each strain measured on motility plates containing 0.1% DMSO (carrier control; closed circles with line) or 1 mM inhibitor F1, F2, F3, or F4 (open symbols; squares, triangles, inverted triangles, and hexagons, respectively), *p < 0.05, ANOVA. SEM, standard error of the mean.