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. 2018 Jul 31;13(7):e0200499. doi: 10.1371/journal.pone.0200499

Fig 5. Effects of 7-ketocholesterol on ATF-2 activity in human umbilical vascular endothelial cells (HUVECs).

Fig 5

HUVECs were pretreated with 50 μM of cholesterol or 7-ketocholesterol for 18 h, incubated with 5 μM p38MAPK inhibitor (SB203580) for 30 min and stimulated with tumor necrosis factor (TNF)-α for an additional 4 h. Western blotting was used to evaluate the nuclear levels of phospho-ATF-2 and ATF-2 as described in the Materials and Methods. Representative blots from three independent experiments are shown.