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. 2018 Jul 13;14(7):e1006185. doi: 10.1371/journal.pcbi.1006185

Fig 2. Examples of the types of miRNA binding sites considered by different candidate site selection methods (CSSMs).

Fig 2

(a) Potential canonical binding site accepted by the PITA, TargetScan (TS), and miRAW CSSMs. Here, the seed region contains a perfect 7mer. (b) Potential non-canonical compensatory binding site accepted by TS and miRAW CSSMs. The missing nucleotide pair in the seed region is compensated by the 9 consecutive pairs starting at position 10—centered pairing requires at least a 4mer at positions 10 to 14. (c) Potential non-canonical centered target site accepted by TS and miRAW CSSMs. The lack of perfect seed matching is compensated by additional consecutive pairs in nucleotides 9 to 12. (d) Potential non-canonical sites accepted only by the miRAW CSSMs. The extended seed region (10 nucleotides) and the inclusion of wobbles allows these scenarios to be considered as potential target sites.