Figure 8.

Immune cell recruitment and antigen uptake induced by SLA/LA. C57BL/6 mice (n = 4–5/group) were injected i.m. in the left tibialis anterior (TA) muscle on Day 0 with AF647-labeled OVA (20 µg; alone or encapsulated in SLA/LA archaeosomes). The right TA of all animals was injected with PBS for use as a negative control. The TAs and draining inguinal lymph nodes (right and left) were collected on Days 1 or 3 following injection. Immune cells were isolated from each tissue, counted and fluorescently stained with an antibody cocktail to differentiate different immune cell populations: B cells, CD8+ T cells, dendritic cells (DC), macrophages, monocytes and neutrophils. The number of cells from the above cell populations was determined per tissue through flow cytometry (A; group means ± standard deviation). The number and immunophenotype of antigen-positive (OVA-AF647+) cells was determined in each tissue (B; group means).