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. 2018 Jul 31;7:e38801. doi: 10.7554/eLife.38801

Figure 6. mig-15 functions in DA neurons.

Figure 6.

(A–D) Representative images of synaptic tiling marker (wyIs446) in the following backgrounds: mig-15(rh148); Ex[Punc-4c::mig-15] (A), mig-15(rh148); Ex[Pmig-13::mig-15] (B), wild type (C) and wild type animals expressing dominant-negative mig-15(K50A) in DA neurons (D). Synaptic domains of DA8 and DA9 are highlighted with green and magenta lines, respectively. Asterisks: DA9 cell body. Arrows: dorsal commissure of DA9. Scale bars: 20 μm. (E) Amino acid alignment of amino-terminal region of MIG-15 and TNIK. A kinase-dead mutation in TNIK (K54A) and corresponding mutation in MIG-15 (K50A) are highlighted in red. (F–I) Quantification of overlap between DA8 and DA9 synaptic domains (F and I), DA9 synaptic domain (G), DA8 asynaptic domain (H) in respective mutant backgrounds. Each dot represents measurements from a single animal. Blue bars indicate mean ± SEM. n.s.: not significant; ***p<0.001; *p<0.05.