Figure 3. Atoh1::En1-CKO mice have abnormal respiratory chemoresponses that cannot be rescued by caffeine.

Normalized changes in tidal volume (T_V) (A), respiratory frequency (V_f) (B), and minute ventilation (V_E) (C and D) during hypoxic challenge (10% O₂, balanced N₂). Atoh1::En1-CKO mice show respiratory repression during hypoxia (B) that cannot be rescued by caffeine (D). Normalized changes in T_V (E), V_f (F), and V_E (G and H) during hypercapnic chemochallenge (5% CO₂, 21% O₂, balanced N₂). Atoh1::En1-CKO mice have attenuated response to hypercapnia (E and F) that cannot be rescued by caffeine (H). Significance was determined using a t-test (2-tailed) at each individual time point, *p<0.0011 (0.05/44 for Bonferroni correction). Error bars represent mean ± SEM.

Figure 3—figure supplement 1. Silencing cerebellar cortex output neurons does not cause abnormal respiratory control.

(A) Mice with silenced Purkinje cells (Pcp2^Cre/+;Slc32a1^Flox/Flox) have slightly more irregular breathing rhythms (iii), but otherwise normal respiratory rhythms in room air. Significance for room air breathing parameters were determine using a t-test (2-tailed). *p<0.05. (B) Pcp2^Cre/+;Slc32a1^Flox/Flox have normal respiratory chemoresponses to hypoxia and hypercapnia. Significance was determined using a t-test (2-tailed) at each individual time point, *p<0.0011 (0.05/44 for Bonferroni correction). Error bars represent mean ± SEM.