Figure 4. P7 Atoh1::En1-CKO mice have abnormal respiratory control.

(A) Representative plethysmography traces from a control and Atoh1::En1-CKO mouse. Example traces of apnea and sigh. (B) One-week-old Atoh1::En1-CKO mice have significantly more apneas (i), longer inspiratory time (T_I) (iii), smaller tidal volume (T_V) (v), slower respiratory rhythms (V_f) (vi), and lower minute ventilation (V_E) (vii). Number of sighs (ii) and expiratory time (T_E) (iv) were not different. Significance for room air breathing parameters were determined using a t-test (2-tailed). *p<0.05. (C) One-week-old Atoh1::En1-CKO mice have enhanced respiratory depression in response to hypoxia through an enhanced decrease in T_V (i) and V_f (ii), resulting in a decreased V_E(iii). There were no significant differences in the respiratory chemoresponses to hypercapnia (iv to vi). Significance was determined using a t-test (2-tailed) at each individual time point, *p<0.01. Error bars represent mean ± SEM.