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. 2018 Jul 4;7:e38455. doi: 10.7554/eLife.38455

Figure 6. Atoh1::HoxA2-CKO mice have normal chemoresponses.

(A) Atoh1::HoxA2-CKO mice have more apneas following sighs (i) and spontaneous apneas (ii) than control littermates and a smaller tidal volume per breath (TV) (vi) resulting in smaller minute ventilation (VE) (Viii). Other breathing parameters were not affected. Significance for room air breathing parameters were determined using a t-test (2-tailed). *p<0.05, **p<0.01. (B) Atoh1::HoxA2-CKO mice have normal respiratory chemoresponses in hypoxia (i to iii) and hypercapnia (iv to vi). Significance was determined using a t-test (2-tailed) at each individual time point, *p<0.0011 (0.05/44 for Bonferroni correction). Error bars represent mean ± SEM.

Figure 6—source data 1. Raw numbers observed surviving offspring.
elife-38455-fig6-data1.xlsx (181.7KB, xlsx)
DOI: 10.7554/eLife.38455.020

Figure 6.

Figure 6—figure supplement 1. Atoh1::HoxA2-CKO mice have abnormal migration of ITR neurons.

Figure 6—figure supplement 1.

(A) In situ hybridization with an Atoh1 probe confirms loss of Atoh1 from the ITR in Atoh1::HoxA2-CKO E14.5 embryos. (B) X-gal stain in Atoh1LacZ/+ and Atoh1::HoxA2-CKO E14.5 embryos to visualize Atoh1-dependent cells in the developing brain. (C) Serial sections at the level of the ITR visualize an abnormal location of ITR neurons at the lateral site of the trigeminal motor neuron in Atoh1::HoxA2-CKO E14.5 embryos. Abbreviations: rRL, rostral rhombic lip; cRL, caudal rhombic lip; ITR, intertrigeminal region; RTN, retrotrapezoid nucleus.
Figure 6—figure supplement 2. Atoh1::HoxA2-CKO mice survive the neonatal period.

Figure 6—figure supplement 2.

(A) Ratio of surviving offspring from Atoh1LacZ /+; HoxA2::CreTG/+ x Atoh1Flox/Flox cross: 9/85 (10.6%) surviving pups were Atoh1::HoxA2-CKO mice (B) Ratio of surviving offspring from Atoh1Flox/LacZ; HoxA2::CreTG/+ x Atoh1Flox/Flox cross: 18/41 surviving pups (43.9%) were Atoh1::HoxA2-CKO mice. Green text indicates Atoh1::HoxA2-CKO mice. These crosses indicate Atoh1::HoxA2-CKO mice are not neonatal lethal and that the HoxA2::CreTG allele is linked to the Atoh1 allele.