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. 2018 Jun 20;14(7):1686–1696. doi: 10.1080/21645515.2018.1480284

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© 2018 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — TLR2 agonists induce chemokine expression in skeletal muscle cells and human foreskin fibroblasts, but not dermal microvascular endothelial cells. Human skeletal muscle (SkMC), human foreskin fibroblast (HFF), and dermal microvascular endothelial (HMEC-1) cell lines were stimulated with TLR2 agonists and quantified for CCL20 and CXCL6 induction by ELISA. TLR2/6 agonists (PAM₂CSK₄ and DBS-2-217C) induced CCL20 and CXCL6 expression in both SkMCs and HFFs, but not HMEC-1 cells. None of the cell lines responded to the TLR1/2 agonist PAM₃CSK₄. Chemokine release assays were performed on triplicate samples. Means and standard deviations are shown.