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Chemotaxis of PBMCs toward TLR2/6- stimulated human foreskin fibroblasts. HFFs stimulated with TLR2/6, but not TLR1/2 agonists elicited functional chemotactic responses from lymphocytic populations in human PBMCs. Resting, adherent fibroblasts were stimulated with graded concentrations of agonists for 24 h in a chemotaxis plate. Chemotaxis of freshly isolated PBMCs was quantified by flow cytometry using lineage-specific antibodies. Means and SD on triplicate samples are shown.
