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. 2018 Jul 31;7:e36740. doi: 10.7554/eLife.36740

Key resources table.

Reagent type
(species) or resource		 Designation Source or reference Identifiers Additional information
Antibody Mouse Anti-alpha-Tubulin
Monoclonal Antibody,
Unconjugated, Clone
DM1A		 Sigma-Aldrich T9026;
RRID:AB_477593 		(1:500)
Antibody Anti-beta-Catenin
antibody produced in
rabbit		 Sigma-Aldrich C2206;
RRID:AB_476831 		(1:300)
Antibody Anti-Histone, H1 + core
proteins, clone
F152.C25.WJJ antibody		 Millipore MABE71;
RRID:AB_10845941 		(1:300)
Antibody anti-NvaPKC custom
peptide antibody
produced in rabbit		 Bethyl labs;
Salinas-Saavedra et al. (2015) 		Stored at MQ
Martindale's lab;
(1:100)
Antibody anti-NvLgl custom
peptide antibody
produced in rabbit		 Bethyl labs;
Salinas-Saavedra et al. (2015) 		Stored at MQ
Martindale's lab;
(1:100)
Antibody anti-NvPar-1 custom
peptide antibody
produced in rabbit		 Bethyl labs;
Salinas-Saavedra et al. (2015) 		Stored at MQ
Martindale's lab;
(1:100)
Antibody anti-NvPar-6 custom
peptide antibody
produced in rabbit		 Bethyl labs;
Salinas-Saavedra et al. (2015) 		Stored at MQ
Martindale's lab;
(1:100)
Antibody Goat anti-Mouse IgG
Secondary Antibody,
Alexa Fluor 568		 Thermo Fisher Scientific A-11004;
RRID:AB_2534072 		(1:250)
Antibody Goat anti-Rabbit IgG
Secondary Antibody,
Alexa Fluor 647		 Thermo Fisher Scientific A-21245;
RRID:AB_2535813 		(1:250)
Antibody Sheep Anti-Digoxigenin
Fab fragments Antibody,
AP Conjugated, Roche		 Roche 11093274910;
RRID:AB_514497
Other Alexa Fluor 488 Phalloidin Thermo Fisher Scientific A12379;
RRID:AB_2315147 		(1:200)
Other Texas Red Streptavidin Vector Laboratories SA-5006,
RRID:AB_2336754 		(1:200)
Other DAPI (4',6-Diamidino-
2-Phenylindole,
Dihydrochloride)		 Thermo Fisher Scientific D1306;
RRID:AB_2629482 		(0.1 µg/µl)
Chemical
compound, drug		 Protein kinase Cζ
pseudosubstrate,
myristoyl trifluoroacetate
salt		 Sigma-Aldrich P1614
Chemical
compound, drug		 1-Azakenpaullone Sigma-Aldrich A3734
Chemical
compound, drug		 Biotinylated Dextran
Amine-Texas Red		 Vector Laboratories SP-1140;
RRID:AB_2336249
Chemical
compound, drug		 Dextran, Alexa Fluor
488; 10,000 MW, Anionic,
Fixable		 Thermo Fisher Scientific D22910
Chemical
compound, drug		 Dextran, Alexa Fluor
555; 10,000 MW, Anionic,
Fixable		 Thermo Fisher Scientific D34679
Chemical
compound, drug		 Dextran, Alexa Fluor
647; 10,000 MW, Anionic,
Fixable		 Thermo Fisher Scientific D22914
Chemical
compound, drug		 Dextran, Cascade Blue,
10,000 MW, Anionic, Lysine
Fixable		 Thermo Fisher Scientific D1976
Deposited Data Nematostella vectensis
genome assembly 1.0		 JGI https://genome.jgi.doe.gov/Nemve1/Nemve1.home.html
Biological sample Nematostella vectensis Whitney Laboratory for
Marine Bioscience, FL, USA.		 RRID:SCR_005153
Sequence-based
reagent		 Genomic PCR Nvpar-6 and
dnNvpar-6: F-AAAACCAC
CATCAGCCGAGTCA;
R-TATTGATAGAATACCAGTCTCA		 NEMVEDRAFT
_v1g233358
Sequence-based
reagent		 sgRNAs Nvpar-6:
1-GGATGTTGCCGACTCGCAGT;
2-GGAGAAGGCGAACTCGTCTG;
3-GGATAACCCTGTGCCAGTCA		 CRISPRevolution sgRNA;
Synthego		 NEMVEDRAFT
_v1g233358
Sequence-based
reagent		 dnNvpar-3: F-ATGATGAAGGTTGTAGT;
R-TGCGCCCGATTCGAATCCATCT		 NEMVEDRAFT
_v1g240248
Sequence-based
reagent		 sgRNAs Nvpar-3:
1-GGGTGTTCGAGGGACGCGAT;
2- GGGCAGGTTTATCCCGAAGG;
3- ACCAACGAUCUAGAUCCAGU		 CRISPRevolution sgRNA;
Synthego		 NEMVEDRAFT
_v1g240248
Sequence-based
reagent		 Genomic PCR Nvpar-3:
F-GTAGACGGGACTGGTTTGGA;
R-AGGGACAGGTTGCTCCTTTT		 NEMVEDRAFT
_v1g240248
Sequence-based
reagent		 dnNvpar-1:
F-AATATAAACTATGAACTTAACG;
R-TTAAAGTTTTAATTCATTTGCA		 NEMVEDRAFT
_v1g 139527
Sequence-based
reagent		 sgRNAs Nvsnail-A:
2-GGGGCCGGTAATGACGCGCG;
3-GGCGTAGAGTCACACCGCAA;
4-GGCGATGATATCGAGCTCGG;
5-GGGCATCTTGAGTGCACCCA		 CRISPRevolution sgRNA;
Synthego		 NEMVEDRAFT
_v1g240686
Sequence-based
reagent		 sgRNAs Nvsnail-A:
1-GGGCTCTCTTGCTCCGTAAC;
6-GGGTTTCCTGGCGCTGGGAT		 CRISPRevolution Modified
sgRNA; Synthego		 NEMVEDRAFT
_v1g240686
Sequence-based
reagent		 Genomic PCR Nvsnail-A (full length):
F-ATGCCCCGCTCGTTTCTAG;
R-TCCTTGTGACGGGCAGCC		 NEMVEDRAFT
_v1g240686
Sequence-based
reagent		 sgRNAs Nvsnail-B:
1-GGAAGAGGATGTGAGGTTTT;
2-GAGATGATATTAGGCTGGTG;
4-GAAAAGCTGTACGACTCCTT;
5-GGGCATCTTGAGAGCGCCCA		 CRISPRevolution sgRNA;
Synthego		 NEMVEDRAFT
_v1g236363
Sequence-based
reagent		 sgRNAs Nvsnail-B:
3-GGGTGAAGACTAAGACAGAG;
6-GGGCGATGAATCGTGTTTAA		 CRISPRevolution Modified
sgRNA; Synthego		 NEMVEDRAFT
_v1g236363
Sequence-based
reagent		 Genomic PCR Nvsnail-B (full length):
F-ATGCCGAGGTCCTTCCTGG;
R-GCAGAGATTTTGCCGACACAT		 NEMVEDRAFT
_v1g236363
Recombinant
DNA reagent		 pSPE3-mVenus Roure et al. (2007) Gateway vector
Recombinant
DNA reagent		 pSPE3-mCherry Roure et al. (2007) Gateway vector
Recombinant
DNA reagent		 pSPE3-Nvpar-6-mVenus Salinas-Saavedra et al. (2015)
Recombinant
DNA reagent		 pSPE3-Nvpar-3-mVenus Salinas-Saavedra et al. (2015)
Recombinant
DNA reagent		 Nvß-catenin expression
constructs		 Wikramanayake et al. (2003);
Röttinger et al., 2012
Recombinant
DNA reagent		 Nvsnail-A backbone to
generate expression
constructs		 Magie et al. (2007)
Software,
algorithm		 Fiji (ImageJ) NIH http://fiji.sc
Software,
algorithm		 Imaris 7.6.4 Bitplane Inc.
Software,
algorithm		 CRISPRscan Moreno-Mateos et al. (2015) http://www.crisprscan.org/
Software,
algorithm		 SPSS IBM