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. 2018 May 11;11(2):1–5. doi: 10.1080/19420889.2018.1467188

Figure 1.

Figure 1.

Quantitative RT-PCR analysis of PvRbohA expression and root growth parameters in Phaseolus vulgaris. A representative wild-type P. vulgaris germinating seedling showing (A) a radicle (from 2-day-old seedling) and (B) ILR and LR zones of the root (from 4-day-old seedling). (C) RT-qPCR expression analysis of PvRbohA from mRNA isolated from radicles, ILR zones, and LR zones of wild-type P. vulgaris seedlings. Transcript accumulation was normalized to the expression of the Ef1α and IDE reference genes. The statistical significance of differences between the different root zones was calculated by ANOVA and Tukey's Multiple Comparison Test, where different letters indicate significance differences (P < 0.001). P. vulgaris composite plants containing transgenic hairy roots were analyzed at 10 days post transplantation. (D) Primary root length and (E) lateral root density in control and PvRbohA-RNAi roots. The statistical significance of the differences between control and RNAi root samples was determined using an unpaired two-tailed Student's t-test (*P<0.05). The reported values represent three biological replicates (C; n > 9, D, E; n > 21). Error bars represent the means ± SEM. Scale bar: A, 5 mm; B, 2 mm. ILR zone, inter lateral root zone; LR zone, lateral root zone.