Figure 2.

Expression analysis of the PvRbohA promoter driving the β-glucuronidase reporter gene in transgenic P. vulgaris roots. The spatio-temporal pattern of PvRbohA activity was examined in transgenic hairy roots harboring the promoter::GUS-GFP construct incubated in GUS as a substrate. (A-H). Representative images showing promoter activity detected by GUS staining during different stages of LR formation in transgenic roots. (A-B) LR pre-initiation, (C-D) LR primordium initiation, and (E-H) LR emergence. Scale bar, A-E, H: 200 µm; F-G 500 µm. LR: lateral root.