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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: JPEN J Parenter Enteral Nutr. 2018 Jan 31;42(7):1156–1167. doi: 10.1002/jpen.1053

Figure 4.

Figure 4

Effects of Clostridium difficile (CD) on tight junction protein and an anion exchanger expression in proximal colon. Mice were treated as described in Figures 1 and 2. Proximal colon was collected and embedded in optimal cutting temperature medium (OCT) for histology on days 1 and 5 after CD exposure. (A) Occludin (red), zona occludin-1 (ZO-1; green), (B) claudin-3 (green), and (C) Na+/H+ exchanger isoform 3 (NHE3; green) were visualized by immunohistochemistry in sections of proximal colon frozen in OCT. A selected area was cropped and enlarged. All images were acquired using a 40 × objective. Images are representative of at least replicate images captured per mouse in 3–6 mice per treatment group. FP, Faecalibacterium prausnitzii; PS, potato starch.