Fig. 2.
LMWF5A decreases mRNA for IL-6, CXCL-10, IL-12, and specific Toll-like receptors (TLR3 & TLR7) in PMA-differentiated THP-1 cells stimulated with LPS (100 ng/ml). Total RNA was isolated from THP-1 cells treated with saline or LMWF5A (N = 3). RT-qPCR was performed using a profiler array kit specific for the Toll-like receptor signaling pathway (Qiagen, Hilden, Germany). Using the ΔΔCT method, relative fold changes in the LMWF5A-treated differentiated THP-1 cells were quantified versus saline-treated differentiated THP-1 cells. All RT-qPCR data was normalized to internal housekeeping genes included in 96-well array. Data is presented as fold change ± SD. All presented data is significant (α < 0.05) versus saline control.