Table 1.

Kinetic parameters of Fab–insulin interactions at 25 °C.

Analyte	Kd(M)	kon(M−1s−1)	koff(s−1)
Wild type	2.22 × 10−10	(2.48 ± 0.01) × 106	(5.49 ± 0.01) × 10−4
R3-mutant	5.49 × 10−11	(8.79 ± 0.02) × 106	(4.83 ± 0.01) × 10−4
R5-mutant	2.03 × 10−12	(7.19 ± 0.05) × 106	(1.46 ± 0.01) × 10−5
K5-mutant	8.04 × 10−12	(5.44 ± 0.04) × 107	(4.37 ± 0.01) × 10−4
D5-mutant	9.69 × 10−10	(7.64 ± 0.04) × 105	(7.40 ± 0.02) × 10−4
E5-mutant	1.52 × 10−9	(2.79 ± 0.01) × 105	(4.24 ± 0.01) × 10−4

The values obtained by global fitting are the means ± SE.