Figure 5. Functional identification of preBötC via microinjections of DLH.
Unilateral injection of DLH (10 mm) into the VLM of urethane‐anaesthetized, paralysed adult rats when measuring instantaneous respiratory frequency (inst. freq., breaths min–1) from ∫PN. A, unilateral injection of DLH into the preBötC produces a robust increase in respiratory frequency. B, injection of DLH 200 μm rostral to the preBötC (presumably in the BötC) reduced the inspiratory frequency. C, DLH injection 300 μm caudal to the preBötC had minimal effect on inspiratory rhythm.