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. 2018 Jul 31;8:11488. doi: 10.1038/s41598-018-29852-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Specific reduction of S100P by siRNA leads to significant impairment in invasive abilities of Jeg-3 and Bewo trophoblast cells. Jeg-3 (A) and Bewo (B) cells were treated with different S100P siRNAs (siRNA4 and siRNA6) or control siRNAs for 48 hours prior to starvation with low serum containing medium. 24 hours later, cells were seeded in Boyden chambers previously coated with matrigel and incubated for 16 hours prior to fixation and staining using the Diffquik histochemical kit for labelling of both nuclei and cytoplasm. (A,B) 5 random fields were quantified for each chamber. Data is presented as means ± SEM of 4 independent experiments relative to controls (percentage) from 4 replicate wells for each set of conditions. ***P < 0.0001 compared to control and mock treated (one way- ANOVA). Images of representative fields of motility/invasion assays were taken with the EVOS XL Cell Imaging System at x20 magnification.