Table 1.

Reduction of S100P in trophoblast cells leads to increases in the number of focal adhesions per cell.

Cell lines	Percentage focal adhesions per cell ± SEM (n = 50)	P-valuea	P-valueb
Jeg3 control	100 ± 2.22
Jeg3 treated with S100P siRNA4	177.08 ± 4.54	P < 0.0001
Jeg3 treated with S100P siRNA6	190.38 ± 3.67	P < 0.0001	0.4916
Bewo control	100 ± 1.85
Bewo treated with S100P siRNA4	166.16 ± 3.04	P < 0.0001
Bewo treated with S100P siRNA6	171.57 ± 3.99	P < 0.0001	0.9033

Jeg-3 and Bewo control cells, as well as cells treated with different S100P targeted siRNA for 72 hours were fixed and stained for paxillin and actin after seeding on fibronectin-coated coverslips. Data shown are means ± SEM corresponding to the average number of focal adhesion-containing paxillin observed per cell, presented as percentage of control. Untreated and mock treated controls (data not shown) were found to be not statistically significant (P > 0.05).

^aP-value obtained from one-way ANOVA where total number of focal adhesions present in Jeg-3 or Bewo control cells were compared to previously S100P siRNA-treated counterparts.

^bP-value obtained from one-way ANOVA where total number of focal adhesions present in Jeg-3 or Bewo cells treated with siRNA4 were compared to siRNA6-treated counterparts.