Table 2.

Reduction of S100P in trophoblast cells leads to increases in the number of focal adhesions per migrating cell.

Cell lines	Number of focal adhesions per cell ± SEM (n = 15)	P-valuea	P-valueb
Jeg-3 control	100 ± 1.98
Jeg-3 mock control	106.13 ± 2.22	0.2858
Jeg-3 treated with S100P siRNA4	160.65 ± 3.55	P < 0.0001
Jeg-3 treated with S100P siRNA6	166.87 ± 3.06	P < 0.0001	0.9201

Jeg-3 control cells, as well as mock treated cells and cells incubated with different S100P targeted siRNA for 72 hours were grown until fully confluent prior to wound healing and grown for a further 16 hours before being fixed and stained for paxillin and actin. Data shown are means ± SEM corresponding to the average number of focal adhesions containing paxillin observed per cell at the leading front, presented as percentage of control. Untreated and mock treated controls (data not shown) were found to be not statistically significant (P > 0.05).

^aP-value obtained from one-way ANOVA where total number of focal adhesions presents in Jeg-3 or Bewo control cells were compared to previously S100P siRNA-treated counterparts.

^bP-value obtained from one-way ANOVA where total number of focal adhesions present in Jeg-3 or Bewo cells treated with siRNA4 were compared to siRNA6-treated counterparts.