Figure 4.

Itch^−/−IL-4^−/− mice exhibit comparable bacterial control and alveolar macrophages as IL-4^−/− control mice. IL4^−/− and Itch^−/−IL4^−/− mice were infected intranasally with 10³ CFU K. pneumoniae. (a) Survival (n=7–8, combined from two independent experiments), (b) Weight loss (n=10–11) and (c) bacterial colony-forming units (CFU) in lung homogenate on day 3 post infection (n=8, compiled from three independent experiments). (d) BAL cytokines were quantified using the Quansys 16-cytokine enzyme-linked immunosorbent assay array (n=6, compiled from three independent experiments). (e) Myeloid cells were identified from lung single-cell suspensions using flow cytometry. Neutrophils were CD45⁺SiglecF⁻CD11b⁺Ly6G⁺, eosinophils were CD45⁺SiglecF⁺CD11c⁻, monocytes were CD45⁺, SiglecF⁻, Ly6G⁻CD11b⁺Ly6C⁺ and alveolar macrophages were CD45⁺CD11c⁺CD11b^loSiglecF⁺ (n=5–8, compiled from three independent experiments). Dots represent individual mice. Significance was determined for survival, weight loss and cytokine/bacterial burden/myeloid cell populations as follows: Log-rank test, two-way analysis of variance and unpaired t-test were used, respectively.