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. 2018 Jul 31;8:11474. doi: 10.1038/s41598-018-29847-z

Figure 4.

Figure 4

TNF affects the cell cycle and the expression of core-clock genes in HL. Gene expression, supernatant concentration of TNF and cell cycle analysis for the three cell lines (WT) after three days of stimulation with 50 ng/mL of recombinant human TNF, 0.1 uM dexamethasone and 25 uM of Forskolin. (A) Gene expression qPCR data (n = 3, mean ± SEM). (B) Supernatant concentration of TNF (n = 3, mean ± SEM). (C) Cell cycle analysis for the three cell lines (LCL-HO, the HL cell line HD-MY-Z and the HL cell line L-1236) (n = 3, mean ± SEM). (D) Gene expression data for Bmal1, Per2, Tnf, Cry1, Cry2 and Rev-erbα of LCL-HO, HD-MY-Z and L-1236 cell lines after shRNA KD (n = 3, mean ± SEM). The corresponding shRNA KD for the specific cell is represented under each plot. *p < 0.05, **p < 0.01, ***p < 0.001.