Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 31;8:11474. doi: 10.1038/s41598-018-29847-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Tnf and core-clock genes affected cell migration in the HL cell line HD-MY-Z. (A) Migration properties of pLKO.1 empty vector and KD HD-MY-Z cell lines (shBmal1, shTNF, shPer2 and shRev-erbα), measurements were obtained using a scratch wound assay for the IncuCyte S3 Live Cell System Analysis. Quantification was performed by measuring the relative wound confluence over the course of 34 hours. (n = 3, mean ± SEM). (B) Partial representation of the scratch wound assay for all HD-MY-Z KD derived cell lines in the course of 12–22 hours. Images were obtained with the IncuCyte S3 Software. Yellow mask indicates the wound border locations. Blue mask indicates the initial scratch wound area. (C) A model for the bi-directional role of TNF and the core-clock in regulating the molecular and cellular characteristics in HL. TNF regulates gene expression, cell cycle and apoptosis by interacting with the core-clock elements Bmal1, Per2 and Rev-erbα (orange circles). The grey area represents stimulatory effects of TNF on KD cell lines. TNF stimulation differentially affects the cell cycle phases of the KD cell lines, as illustrated by the different sizes and colour of each blue square (compared to pLKO.1). Cellular apoptosis is influenced by TNF stimulation in shRNA cell lines leading to its activation (shBmal, shRev-Erb) or inhibition (shPer2).