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. 2018 Jul 31;8:11493. doi: 10.1038/s41598-018-29752-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence of processed ‘en face’ optical coherence tomography angiography (OCTA) demonstrates vessels at different corneal depths. A series of coronal ‘en face’ corneal sections from subepithelial (a) to mid stroma layer (i) of rabbit 5 at week 6, every 20 microns interval, depicting corneal vessels of the same rabbit 5 at week 6 as in Fig. 2. The red Δ indicates the same vessels in area 1 of Fig. 2, which are present in superficial layers (d,e) whereas the fan-like vessels are present in the deeper layers (f,g,h). Note, discontinuity of the vascular network and overlapping of same vessels in different frames caused by unparallel segmentation, which stems from the natural three dimension growth of vessels and steep rabbit corneal curvature.